Super-Resolution
THOR - Nikon A1 laser scanning, structured illumination and TIRF (Porter B047A)
Lasers:
Lauch: LUNV6, high-output.
Excitation at: 405, 445, 488, 515, 561, 647 (nm)
Emission (Chroma) and molecules imaged:
- Emission filter: 425-475 (nm): DAPI, Hoechst, DyLight-405, Alexa405, etc.
- Emission filter: 467.5-502.5 (nm): CFP, cerulean, etc.Ìý
- Emission filter: 500-550 (nm): Alexa488, FITC, EGFP, Opal520, etc.Ìý
- Emission filter: 521.5-554.5 (nm): YFP, Venus, etc.Ìý
- Emission filter: 575-625 (nm): Alexa555, 568, 594, TxRed, mRFP, mCherry, TdTomato, Opal570, etc.Ìý
- Emission filter: 650-720 (nm): Alexa647, Cy5, Opal620, etc.Ìý
- DIC imaging possible (laser scanning confocal only)
Filters for widefield:
DAPI, GFP, RFP, Brightfield
N-SIM certified objectives:
- 100x 1.49NA OIL SR Apo TIRF WD 0.12 (mm)
- 60X 1.27NA Water SR Plan Apo WD 0.17 (mm)
Standard objectives:
- 4x 0.2NA Plan ApoÌýλ WD 20 (mm)
- 10x 0.45NA Plan Apo λ WD 4 (mm)
- 20x 0.75NA Plan Apo VC λ WD 1 (mm)
- 40x 0.6NA S Plan Fluor ELWD 3.6-2.8 (mm)
- 40x 0.95NA Plan Apo λ WD 0.25-0.17 (mm)
Available upon request:Ìý
- 60x 1.40NA Plan Apo OIL VC λ WD 0.13 (mm)
Laser Scanning Confocal:
- 2 x GaAsP high-sensitivty PMTs (CFP, GFP, YFP, RFP)
- 2 x multi-alkali PMTs (DAPI, far-red)
N-SIM:
Ìý512 X 512 16 bit
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- TOKAI-HIT Cell Incubator 1 x 35 (mm) dish or chamber side (coverslip bottom)
- Motorized X, Y, Z and separate Mad City Labs Piezo Z
- Multi-point, time-lapse, optical sectioning, large-area montage with stitching
- Increase power over imaging depth
- Ìý5.21.03; 64 bit
